Improvements in the extraction of milk phospholipids from beta-serum using ultrasound prior to tertiary amine extraction

We report improvements in the extraction of milk phospholipids (MPLs) from beta-serum, a dairy byproduct, by applying ultrasound prior to tertiary amine extraction. Three acoustic intensities (15.53 ± 1.20, 31.76 2.46, or 44.56 3.47 W cm−2) were applied for 4 min before (N,N-dimethylcyclohexylamine, CyNMe2). The extracted lipids fractionated solid phase-microextraction, and recovered MPLs quantified HPLC-CAD. An intensity cm−2 followed CyNMe2 (12/1, solvent ratio) yielded 69.67 3.45% MPLs, while only 7.57 0.59% without ultrasound. fraction was made phosphatidylinositol (32%), phosphatidylethanolamine (30%), sphingomyelin (37%). Scanning electron images particle size revealed significant disruption complex arrangement between membrane proteins which may help release into aqueous medium.